The hill people and the Mexicans arrived on the same day. It was a Wednesday, early in September 1952. The Cardinals were five games behind the Dodgers with three weeks to go, and the season looked hopeless. The cotton, however, was waist-high to my father, over my head, and he and my grandfather could be heard before supper whispering words that were seldom heard. It could be a "good crop."They were farmers, hardworking men who embraced pessimism only when discussing the weather and the crops. There was too much sun, or too much rain, or the threat of floods in the lowlan
vt.&n.欢迎a.受欢迎的offervt.&n.提供;贡献be about to...(不久)就要,即将trainingn.训练;培养programn.规划;(教学)大纲generala.一般的,普遍的basica.基本的,基础的coursen.课程;过程;疗程biologyn.生物学devotevt.贡献medicala.医学的,医药的anatomyn.解剖学;解剖physiologyn.生理学biochemistryn.生物化学termn.学期;期限;术语internala.内服的;内部的surgeryn.外科学,外科and so on等等,诸如此类clinicala.临床的have to不得不,只好in addition另外follow-upa.&n.接着的,后续的advanceda.高级的;先进的besides...
PCR引物设计的目的是为了找到一对合适的核苷酸片段,使其能有效地扩增模板DNA序列。因此,引物的优劣直接关系到PCR的特异性与成功与否。要设计引物首先要找到DNA序列的保守区。同时应预测将要扩增的片段单链是否形成二级结构。如这个区域单链能形成二级结构,就要避开它。如这一段不能形成二级结构,那就可以在这一区域设计引物。现在可以在这一保守区域里设计一对引物。一般引物长度为15~30碱基,扩增片段长度为100~600碱基对。让我们先看看P1引物。一般引物序列中G+C含量一般为40%~60%。而且四种碱基的分布最好随机。不要有聚嘌呤或聚嘧啶存在。否则P1引物设计的就不合理。应重新寻找区域设计引物。同时引物之间也不能有互补性,一般一对引物间不应多于4个连续碱基的互补。...
This is what happened. On the night that the worst heat wave in northern New England history finally broke-the night of July 19-the entire western Maine region was lashed with the most vicious thunderstorms I have ever seen. We lived on Long Lake, and we saw the first of the storms beating its way across the water toward us just before dark. For an hour before, the air had been utterly still. The American flag that my father put up on our boathouse in 1936 lay limp against its pole. Not even its hem fluttered. The heat was like a solid thing, and it seemed as deep as sull
1 With a gentle sigh the service tube dropped a message capsule into the receiving cup. The attention bell chimed once and was silent. Jason dinAlt stared at the harmless capsule as though it were a ticking bomb. Something was going wrong. He felt a hard knot of tension form inside of him. This was no routine service memo or hotel munication, but a sealed personal message. Yet he knew no one on this planet, having arrived by spacer less than eight hours earlier. Since even his name was new-dating back to the last time he had changed ships- there could be no personal mes
Sao Paulo Airport, Brazil, 1991 With a POWERFUL KICK FROM ITS twin turbofan engines, the sleek executive jet lifted off the runway and shot into the vaulted skies above Sao Paulo. Climbing rapidly over the biggest city in South America, the Learjet soon reached its cruising altitude of thirty-nine thousand feet and raced toward the northwest at five hundred miles an hour. Seated in a fortable rear-facing chair at the back of the cabin, Professor Francesca Cabral peered wistfully out the window at the cottony cloud cover, already missing the smog cloaked streets and sizz
日期:2009-6-22 22:37:00 人类历史上许多未解之谜,像史前文明的形成、断裂,大洪水的发生,十日并出,太阳消失,这一切都是月亮造成的!这是无法回避的事实。大约在15000多年以前,一艘来自宇宙深处的外星人飞船——月亮宇宙飞船,突然拐了一个弯,驶进了太阳系,并降临地球近地轨道,悬浮在中国西北部地区的上空,低得仿佛一踮脚就可以摸得着,传说中的“神”驾临了地球。这不是科幻,而是事实。在中国西南瑶族地区,有一则古老的传说:在远古的时代,天上只有太阳和星星,却看不见月亮,那时的夜空漆黑如墨,每当夜晚降临,大地上就被恐惧笼罩,人类和其他动物一样都躲进自己的巢穴。有一天晚上,天空中突然出现了一个热烘烘、七棱八角的大山一样的东西,它不圆不方,像一块巨大的石头,放射着毒热的光芒。瑶族人的这则传说讲的就是月亮的来由,月亮是在某一天突然出现在地球上空的,当时已经有了人类。瑶族的这则传说有几分...
补疑 三字诀 修真诀,要心传,无师指,是枉然。有渐修,有顿悟,安勉分,两路作。上智少,中士多,设渐法,中士科。先修命,后修性,渐归顿,是真行。有药物,有火候。理幽深,当细究。安坤炉,立乾鼎,配阴阳,头一等。也用刚,也用柔,两相应,是丹头。活中死,死中活,死活明,万缘脱。真藏假,假出真。会运法,出凡尘。我是我,它是它,知的了,不遭魔。害生恩,恩生害,不被瞒,揭覆盖。火上焰,水下流,颠倒过,住瀛洲。道心生,人心灭,黑中白,光皎洁。空即色,色即空,色空祛,大神通。有法相,有色相,色相离,法相亮。有逆运,有顺行,顺则死,逆则生。有还丹,有大丹,两事理,细研钻。须持心,须炼己,固本根,利出否。分内外,辨主宾,常返照,旧换新。朋来坤,朋丧艮,转枢机,无危困。龙本恶,虎本颠,二物伏,性情绵。戊应外,己定内,变刀圭,不昏昧。水中金,火中木,锻炼成,本原复。上弦金,下弦水,金...
1 基因上游(3500)AACTGACAAGGGAGCCTGGTACATGGTACGAGCAGCAGTAGTAGTCAGAATCCGTGAGAAATCACTAATACATGCTGCGGTTGTCTTTCTGTTAATGGAATGAGGATAGTGATTTGGCTTGGCCGCTGAGATCGTACAGCGGGCAATACGGAATATCCAGGAGCGTGATACGGTGGGTGGATTTGTTGATAGACAGTAACTCGGATCTCCCTCTTCCTCCCAATCCCTGGGATCAGAACAAGGTCGTCCGTCTTGGGATATACCGAGCAGGCGCGGTTGATATCAAACCTCTAACCATAAGGCCAGCGCGCAGTGATATATTGATGGCATGACGCTGCTTGCACAAATTCATGTTTCCAGCGGTGGGAGCAGCCAAATGGCCGTGAACTCATCACCTTGCCCGATAGCACAACAGCTAATAGAACATGGTGGACTAGACCCCTTTGCCTCTTGATGCGATGCCGCGTCGTGTCTCCAGCAAAACTCCACAATTCAATGCCCACCTCCATGCGAAGTCAGTCTGGTCAGCCAATGTTCGCCAAAGCACTGGCTGCGGCGCTAGCTGGG
边缘营销——游走在主流营销的之外的价值营销(一) 什么是边缘营销?顾名思义那就是主流营销之外的一种营销方式。要搞明白什么是边缘营销就要搞清楚什么是主流营销。现在的主流营销无非就是传统营销和已经形成主流的网络营销等,传统的市场营销策略是由迈卡锡教授提出的4P组合,即产品、价格、渠道和促进。这种理论的出发点是企业的利润,而没有将顾客的需求放到与企业的利润同等重要的地位上来。而网络的互动性使得顾客能够真正参与整个营销过程,而且其参与的主动性和选择的主动性都得到加强。这就决定了网络营销首先要求把顾客整合到整个营销过程中来,从他们的需求出发开始整个营销过程。据此,以舒尔兹教授为首的一批营销学者提出了4C的市场营销理论,即消费者的需求和欲望、成本、便利和沟通。这也形成了传统主流营销的核心部分,之后的所谓5P、6S的,都未脱离这种理论,只是一种延伸和补充,尚未构成新的营销理论体...
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On July 16, in the aching torpid heat of the South Florida summer, Terry Whelper stood at the Avis counter at Miami International Airport and rented a bright red Chrysler LeBaron convertible. He had originally signed up for a Dodge Colt, a sensible low-mileage pact, but his wife had told him go on, be sporty for once in your life. So Terry Whelper got the red LeBaron plus the extra collision coverage, in anticipation of Miami drivers. Into the convertible he inserted the family-his wife Gerri, his son Jason, his daughter Jennifer-and bravely set out for the turnpike. The